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Li, Jing
- A Modified Protocol for Total Rna Isolation from Different Oil Palm (Elaeis guineensis) Tissues using Cetyltrimethylammonium Bromide
Abstract Views :274 |
PDF Views:95
Authors
Affiliations
1 Hainan Key Laaboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Science, Wenchang, Hainan 571339, CN
2 Department of Environmental Sciences, Lahore College for Women University Lahore, Lahore 54600, PK
3 Institute of Horticultural Sciences, University of Agriculture, Faisalabad, PK
1 Hainan Key Laaboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Science, Wenchang, Hainan 571339, CN
2 Department of Environmental Sciences, Lahore College for Women University Lahore, Lahore 54600, PK
3 Institute of Horticultural Sciences, University of Agriculture, Faisalabad, PK
Source
Current Science, Vol 116, No 3 (2019), Pagination: 479-482Abstract
Extraction of high-quality RNA from oil palm tissues is challenging due to the presence of polysaccharides, polyphenols and other complexes that co-precipitate with RNA. Therefore, isolation of high-quality RNA from oil palm is challenging due to the presence of varying amounts of these constituents in diverse tissues. This communication describes a modified RNA extraction protocol based on the cetyltrimethylammonium bromide (CTAB) method which is useful for extracting high-quality RNA from different oil palm tissues. Total RNA isolation using a modified CTAB protocol was compared with two different methods, a conventional TRIzol method and the method for RNA isolation from palms (MRIP). Both methods were useful for isolating RNA from leaf tissues; however, they were not effective in isolating RNA from other tissues. The current protocol based on a modified CTAB method was efficient in isolating high-quality total RNA from oil palm fruit tissues, including mesocarp and endosperm, stem, ischolar_main and flower tissues. This modified CTAB-based protocol gave approximately 20-30 μg of total RNA from 150 mg of tissue within 5-6 h.Keywords
CTAB Method, Gene Expression, Oil Palm, RNA Extraction.Full Text
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- Anti-Tumour and Immune Enhancing Activities of MLAA-22379–387 on Acute Myeloid Leukemia
Abstract Views :268 |
PDF Views:83
Authors
Jing Li
1,
Wanggang Zhang
2,
Ju Bai
2,
Bo Zhong
1,
Huiyuan Wang
1,
Yan Geng
1,
Qiaoyan Jin
1,
Juanjuan Hao
1,
Yang Zhang
1
Affiliations
1 Department of Pediatrics, Medical School of Xi’an Jiaotong University, Xi’an Xincheng Xiwu Road 157, Xi’an 710004, Shaanxi Province, CN
2 Department of Hematology, Medical School of Xi’an Jiaotong University, Xi’an Xincheng Xiwu Road 157, Xi’an 710004, Shaanxi Province, CN
1 Department of Pediatrics, Medical School of Xi’an Jiaotong University, Xi’an Xincheng Xiwu Road 157, Xi’an 710004, Shaanxi Province, CN
2 Department of Hematology, Medical School of Xi’an Jiaotong University, Xi’an Xincheng Xiwu Road 157, Xi’an 710004, Shaanxi Province, CN
Source
Current Science, Vol 118, No 6 (2020), Pagination: 892-900Abstract
We have earlier demonstrated that MLAA-22379–387is a novel, acute, monocytic, leukemia-associated antigen epitope in vitro. In this study, the effect and mechanism of MLAA-22379–387on animals have been further examined. We found that tumour weight and volume had significantly decreased in SCID-injected THP-1 mice with MLAA-22379–387treatment for two weeks. MLAA-22379–387induced cytotoxic T lymphocytes (CTL) activity in A549, MCF-7, THP-1, U937 and T2 cells, especially significant CTL activity at effector/target ratio of 50 : 1 in THP-1 cells. The percentage of CD3 + CD8 + T cells had significantly increased, while the percentage of CD4 + CD25 + T cells had significantly decreased in MLAA-22379–387treatment group compared to other groups. Levels of IL-2, IFN-γand IgG had significantly increased, but levels of TGF-βand IL-10 had significantly decreased after MLAA-22379–387vac-cination for two weeks. Thus, we may conclude that MLAA-22379–387treatment effectively improves the immune system, thus indicating tumouricidal capacity in leukaemic mice. These findings highlight the potential application of MLAA-22379–387 as an efficient target for immunotherapy in acute myeloid leukemia.Keywords
Acute Myeloid Leukemia, Anti-Tumour Activity, Immunotherapy, Mice.Full Text
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